Effect of ginseng Shensong Yangxin capsule and metoprolol sustained release tablet on the curative effect and the levels of BNP and HR in elderly patients with coronary heart disease with arrhythmia / 中国生化药物杂志

Objective To study the effect of Snsong Yangxin capsule and metoprolol sustained release tablet on the curative effect and the levels of BNP and HR in the elderly coronary heart disease (CHD) arrhythmia patients. Methods It selects 108 patients with coronary heart disease and arrhythmia in elderly patients were divided into observation group (54 cases) and control group (54 cases). The control group was treated with metoprolol sustained-release tablets, and the observation group was treated with Shensong Yangxin capsule and metoprolol sustained-release tablets. The curative effect of two groups and the changes of BNP and HR levels before and after treatment were observed and compared. Results The treatment effect, times of contraction, frequency of cardiac fibrillation, frequency of defect, load, time, blood pressure and BNP of the observation group were significantly better than those of the control group (P<0.05). Conclusion For elderly patients with coronary heart disease arrhythmia, the treatment of Shensong Yangxin capsule combined with metoprolol sustained release tablets is effective. It can significantly improve the patient's heart rate, restore the blood supply to the heart, improve the blood pressure and BNP, with less adverse reactions, safe and reliable, and worthy of promotion.

Synthesis of new gene-loaded microbubbles serve as gene delivery vehicle applied in reporter gene transfer into cardiac myocytes / 生物医学工程学杂志

To improve the stability and gene-carried capability of gene-attached microbubbles, the method for manufacture of albumin microbubbles was modified and new gene-loaded microbubbles were synthesized by incorporated gene-PEI complex into the shell of microbubbles. Agarose gel electrophoresis and bacteria transformation showed that PEI had the ability to provide the protection of plasmid DNA from ultrasonic degradation. The new gene-loaded microbubbles exhibited excellent acoustical and hemorheological properties. Moreover, they could carry more plasmid DNA than gene-attached microbubbles. beta-galactosidase plasmid transfection into cardiac myocytes was performed by using ultrasound targeted destruction of new gene-loaded microbubbles or gene-attached microbubbles. Gene expression in cardiac myocytes was detected by beta-galactosidase in situ staining and quantitive assay. It was shown that beta-galactosidase activity in cardiac myocytes was enhanced 107-fold by ultrasonic destruction of gene-loaded microbubbles compared with naked plasmid transfection and new gene-loaded microbubbles resulted in 6.85-fold increase in beta-galactosidase activity compared with optimal transfection mediated by gene-attached microbubbles. These results suggested that ultrasonic destruction of the gene-loaded microbubbles can enhance the cardiac myocytes exogenous gene transfer efficiency significantly and new gene-loaded microbubbles is an efficient and safe gene delivery vehicle.

Effect of Danshao Huaxian Capsule on Proliferation of Activated Hepatic Stellate Cells in vivo and in vitro / 中医杂志

Objective:To study on effect of Danshao Huaxian Capsule on proliferation of activated hepatic stellate cells(HSCs)in vivo and in vitro.Methods:(1)In vitro experiment:57 male Wistar rats were divided into a normal group,a model group and a treatment group.The rats of model group and treatment group were made hepatic fibrosis model with complex stimulation of CCl_4,drinking alcohol,high lipids and low protein diet for 8 weeks.The treatment group were treated for 8 weeks with intragastric perfusion of Danshao Huaxian Capsule,1g/kg.At the end of experiment,a part of the rats were used for detection of hepatic functions and hepatic fibrosis degrees,and another part of the rats were used to separate HSCs.Cell cycle percentage was detected with flow cytometry.(2)In vivo experiment:prepare Danshao Huaxian Capsule serum of the rat;separate cultured HSCs of the rat and divide into calf serum group,normal rat serum group and Danshao Huaxian Capsule serum group.5%,10% and 20% of the above serum was respectively added into the cultured HSCs.Primary HSCs proliferation was detected with MTT method.Results:(1)In vitro experiment:The hepatic function,hepatic fibrosis level and HSCs proliferation reduced significantly.(2)In vivo experiment: Danshao Huaxian Capstde serum group could significantly inhibit proliferation of HSCs as compared with calf serum group and normal rat serum group at the same content.The action showed dose-dependence.Conclusion:Danshao Huaxian Capsule can obviously inhibit proliferation of HSCs in vivo and in vitro.

Treating Severe Type Hepatitis with Oral Traditional Chinese Medicine Concomitant with Artificial Liver:Observation of Curative Efficacy / 中国药房

OBJECTIVE:To observe the clinical efficacy of oral traditional Chinese medicine concomitant with artificial liver in the treatment of severe type hepatitis.METHODS:A total of176patients were randomly divided into the treatment group and control group:the control group was treated with conventional internal medicine therapy together with artificial liver,while the treatment group was treated with additional traditional Chinese medicine besides the therapy for the control group.And the course of treatment was2weeks.RESULTS:The cure rates for the treatment group and the control group were45.5%and20.5%,respectively(P

Effects of phospholamban antisense RNA on SR Ca~(2+)-ATPase and [Ca~(2+)]i in rat cardiomyocytes by adeno-associated virus vector / 中国病理生理杂志

AIM: To investigate the effect of phospholamban antisense RNA (asPLB) on the activity of sarco-endoplasmic reticulum (SR) Ca 2+-ATPase, and the change of intracellular free Ca 2+ concentration ([Ca 2+]i) in rat cardiomyocytes by adeno-associated virus(AAV) vector. METHODS: rAAV-asPLB and rAAV-LacZ were constructed by AAV Helper-Free System. RT-PCR and Western blotting were used to determine the mRNA and protein expression of PLB. The activity of SR Ca 2+-ATPase and the [Ca 2+]i were measured. RESULTS: Compared to controls, the PLB mRNA and protein expression reduced in rat cardiomyocytes transfected with rAAV-asPLB. The activity of Ca 2+-ATPase was increased. In rest state, the level of [Ca 2+]i in rAAV-asPLB transfected group was decreased. The level of [Ca 2+]i was increased when induced by isoproterenol. CONCLUSION: rAAV-asPLB vector disrupts the expression of PLB, enhances the activity of Ca 2+-ATPase, reduces the resting [Ca 2+]i and enhances the isoproterenol-induced [Ca 2+]i.